Ensuring environmental safety during express determination of individual components of plant raw materials in aqueous solutions

Abstract

In the paper, using the method of thin-layer chromatography the peculiarities of determining individual components of plant raw materials- flavonoids were investigated. The test samples were solutions of quartzetin, rutin, kaempferol, hyperoside, caffeic acid, luteolin and chlorogenic acid. Cetylpyridinium chloride, Tween 80 and their modifications with propionic acid were used as the mobile phase. The quantitative determination parameters were retention coefficients and retention factors. It was shown that the presence of a modifier in the mobile phase, cetylpyridinium chloride, increases the retention factor for caffeic and chlorogenic acids, and in the mobile phase, Tween 80 decreases it for these substances. It was found that the use of a mobile phase with Tween 80 (C= 1.57 g/l) ensures the identification of the largest number of investigated substances for which the retention factor increases in a number of hyperosides, chlorogenic acid, caffeic acid, rutin (from 3 to 11). It is proposed to use this variant for the development of a method of rapid identification of these flavonoids in natural waters. It was noted that the use of the proposed eco-friendly methodology will contribute to increasing the level of environmental safety of both individual chemical-ecological studies and territories as a whole.